ABSTRACT
Objective To study the expression of protein 94 (GRP94) and C/EBP-homologous protein (CHOP) in myocardial tissue of hypertensive rats and to investigate the effects of amlodipine on endoplasm retieulum stress ( ERS) and ventricular hypertrophy in abdominal aortic banded rats .Methods One hundred and twenty adult male SD rats with criteria were divided randomly into three groups:sham-operated group , abdominal aortic banding ( AAB) and AAB treated with amlodipine (AAB+Aml)groups(n=40).In sham-operated rats, the abdominal aorta was isola-ted but not constricted , while the abdominal aorta were constricted in AAB rats .The AAB+Aml group was treated by abdominal aortic constriction and treated with amlodipine [10 mg/(kg· d)].According to the time of surgery, each group was further divided into 2, 4 and 8-week postoperative subgroups ( n=6 ) .Mean arterial pressure (MAP), the 1eft ventricular mass (LVM) and body mass (BM) were measured and (LVM/BM) was calculated. The morphology of cardiomyocytes was observed by HE staining .The protein level of GRP 94 and CHOP was ana-lyzedbyimmunohistochemistryandwesternblot.Results 1)Withtimeaftersurgery,MAPandLVM/BWof AAB group increased gradually , and they were obviously higher than the sham operation group [ MAP: 2 weeks (144 ±10)vs(118 ±9), 4 weeks (163±8)vs(120±7), 8 weeks(177±10)vs(120±6)mmHg;LVW/BW:2 weeks (2.21±0.17) vs (1.91±0.12), 4 weeks (2.45±0.16) vs (2.01±0.14), 8 weeks (2.68±0.15) vs ( 2.05 ±0.09 ) mmHg;( P<0.05 ) ] .The cardiomyocytes in AAB group were hypertrophic as compared to the sham group.The expression of GRP94 in AAB group increased significantly at 2 weeks post-operation, and reached peak level at 4 weeks after the surgery and was on the decline thereafter .The expression of CHOP and GRP94 in AAB rats were significantly higher than sham group, and reached the peak at the 8 weeks after surger-y.2)Treatment with amlodipine significantly reduced MAP and LVM/BW in AAB rats[(MAP:2 weeks (126± 6) vs (144±10), 4 weeks (125±8) vs (163±8), 8 weeks (128±5) vs (177±10)mmHg;LVM/BW:2 weeks ( 1.94 ±0.15 ) vs ( 2.21 ±0.17 ) , 4 weeks ( 2.13 ±0.08 ) vs ( 2.45 ±0.16 ) , 8 weeks ( 2.18 ±0.10 ) vs ( 2.68 ± 0.15)mg/g;(P<0.05)].The myocardial hypertrophic was alleviated in AAB+Aml group.The level of GRP94 and CHOP in AAB+Aml group was lower than those in AAB rats ( all P<0.05 ) .Conclusions Endoplasmic reticulum stress is involved in the ventricular remodeling caused by hypertension , and use of amlodipine can reduce ventricu-lar hypertrophy in hypertension models possibly by inhibiting endoplasmic reticulum stress via down -regulation of GRP94 and CHOP .
ABSTRACT
Objective To study and analyze the clinical efficacy of psychological intervention combined with amiodarone on the treatment of arrhythmia in elderly patients with coronary heart disease. Methods 90 elderly patients with coronary heart disease and arrhythmia treated in Changshan people's hospital in Quzhou from February 2015 to September 2016 were randomly divided into the control group and the experimental group, 45 cases in each groups. The two groups was given routine treatment, the control group was treated with amiodarone, at this basis the experimental group were given psychological intervention. To pay attention to the psychological status of the patients, strengthen communication and exchanges with them, increase confidence and compliance during the treatment. The clinical efficacy of the two groups was compared and analyzed. Results The effective rate in the experimental group was 93.3%, which was significantly higher than 73.3% in the control group, the difference was statistically significant (P<0.05). After treatment, the heart rate in the experimental group was (68.79±6.23) times/min, which was significantly lower than (92.30±7.52) times/min in the control group, the difference was statistically significant (P<0.05). Conclusion The clinical curative effect is ideal which psychological intervention combined with amiodarone is used on the treatment of senile coronary heart disease arrhythmia , it can significantly improve the clinical symptoms of patients, improve the treatment efficiency, high safety, has clinical significance.
ABSTRACT
Objective To study and analyze the clinical efficacy of psychological intervention combined with amiodarone on the treatment of arrhythmia in elderly patients with coronary heart disease. Methods 90 elderly patients with coronary heart disease and arrhythmia treated in Changshan people's hospital in Quzhou from February 2015 to September 2016 were randomly divided into the control group and the experimental group, 45 cases in each groups. The two groups was given routine treatment, the control group was treated with amiodarone, at this basis the experimental group were given psychological intervention. To pay attention to the psychological status of the patients, strengthen communication and exchanges with them, increase confidence and compliance during the treatment. The clinical efficacy of the two groups was compared and analyzed. Results The effective rate in the experimental group was 93.3%, which was significantly higher than 73.3% in the control group, the difference was statistically significant (P<0.05). After treatment, the heart rate in the experimental group was (68.79±6.23) times/min, which was significantly lower than (92.30±7.52) times/min in the control group, the difference was statistically significant (P<0.05). Conclusion The clinical curative effect is ideal which psychological intervention combined with amiodarone is used on the treatment of senile coronary heart disease arrhythmia , it can significantly improve the clinical symptoms of patients, improve the treatment efficiency, high safety, has clinical significance.